Hepatitis E virus interaction network to establish therapies

Anbieter der Doktorarbeit

Beschreibung der Arbeit

• experimentell

• Molekulare Methoden (z.B. PCR, RT-PCR, Western Blot, Mikroskopiem, etc) • Virologische und Zellkulturbiologische Arbeiten in BSL2 Labor (Transfektionen, Herstellung lentiviraler Vektoren für die Transduktion, Virusinfektionen, etc) • Bioinformatische Analysen (auf Wunsch)

Hepatitis E virus (HEV) infection is the most common cause of acute hepatitis worldwide. HEV, like all viruses, relies on cellular machineries to replicate and spread and therefore, their proteins evolved to specifically interact with host proteins. Naturally, such interactions represent potential entry points for therapeutic approaches. Here, we will use affinity purification followed by mass spectrometry analyses to uncover the interactions of HEV proteins with the cellular proteome. This will provide an HEV-interaction map of unprecedented detail and reveal cellular proteins and pathways that are affected. Proteins and pathways targeted by HEV could serve as viral host or restriction factors and are, therefore, high-priority candidates for drug-based functional screens. This data-driven approach has the potential to identify novel therapeutic options to blunt the replication of HEV.

Rahmenbedingungen

Wöchentliche Laborseminare, Externe und interne CIID und SFB Seminare, Thesis Advisory Commitee (2x).

2 Freisemester

Hiwi-Vertrag über Stipendium

Bei erfolgreichem Verlauf ist eine Publikation in Erstautorenschaft angestrebt

3-4 monatiger Aufenthalt im Labor von Prof. Pichlmair an der TUM in München ist geplant um die Mass Spec Analysen durchzuführen.